2018 Undergraduate Exhibition

Title Presenter Abstract Faculty Sponsor Numbersort descending Location
Assessment of the blood pregnancy-specific protein B test to determine pregnancy in free-ranging elk (Cervus elaphus) Julia Silva Seixas The serum pregnancy-specific protein B test was analyzed as an alternative pregnancy test for free-ranging elks. It used post-mortem uterine examinations as the gold standard. The serum test used was BioPRYNwild, an enzyme-linked immunosorbent assay performed by Biotracking, Inc. Embryos ranged in age from 13 to 111 days (n = 77). The BioPRYN®wild test had a sensitivity of 93-95% and specificity of 91-93%. The positive and negative predicted values were 87-90-% and 95-96% respectively. Jenny Fisher 137
Biophysical and Biochemical Characterization of Mitochondrial Transcription on Chimeric and Authentic Templates Guinevere Vanbuskirk Oliver For years, mitochondria have been promoted as the “powerhouse of the cell,” because the energy they produce is essential to life. Mutations in mitochondrial DNA (mtDNA) or genes required for mtDNA gene expression have been linked to diseases such as cancer, neurodegenerative disorders, cardiovascular disorders, and muscular dystrophies. I did biochemical and biophysical characterization of mitochondrial DNA templates in order to investigate the transcription of mitochondrial DNA and contribute to research on this important organelle. Craig Cameron 138
Age and genetic background influence initial nicotine sensitivity in C57BL/6J and DBA/2J mice Carley Noel Miller This study examined whether age, genetic background, and their interaction influence the relationship between initial behavioral responses to nicotine and later voluntary nicotine consumption. Our data show that age and genetic background interact to influence initial nicotine sensitivity in DBA/2J and C57BL/6J mice. Further, our data reveal a relationship between acute nicotine responses and later consumption. Our results provide a greater understanding of factors that promote nicotine dependence. Helen Kamens 139
Progressive Forms of Agriculture for Downtown State College Alexandria Chomyn

Climate change is a problem that has been accumulating over the past few decades due to a variety of reasons. There’s an opportunity to look at climate change as an opportunity to better the planet as well as individuals’ diets. Implementing green spaces throughout State College that could be used for agricultural purposes and enjoyment. This will provide the resources to eat a more plant-based diet and fix climate change in the meantime.

Stephen Mainzer 14
Cahal Pech: Recreating Ruins with Structure from Motion Mapping Makaela Bigley

Through the use of 3D modeling, structure from motion mapping, and virtual reality, the ChoroPhronesis lab is making archeological sites available to the classroom.  Cahal Pech, a Mayan ruin in Belize, is the test site for using structure from motion mapping to create models of the ruins that can be placed into a virtual reality platform and be used as an educational tool.

Alexander Klippel 140
Visualizing Large Numbers of Genetic Markers in Select Genes in the Genome Browser Luana Soares Goulet     Visualizing SNP locations through the “My Sessions” feature of the Genome Browser allows for targeting areas of the genome for more in-depth research on the genetic impact on any phenotype. SNPs associated with craving in an exploratory dataset are presented in a new track in the Genome Browser. A graphic display of these SNPs aids in rapid recognition of their relation to each other and to genes of interest. David Vandenbergh 141
Cognitive Effects of Active Sitting Zachary Fox Active sitting has gained increasing prevalence in recent years as a means of increasing psychological performance. The present study evaluated physiological measures of body movements that occur across active and passive sitting conditions as well as performance on cognitive tasks, such as verbal, spatial, and motor tasks. Contrary to popular belief, findings indicate that active sitting provided no benefit on verbal and motor tasks, and may lead to performance decrements on spatial tasks. Praveen Veerabhadrappa 142
Frustrated Lewis Pairs: A New Approach to Pollution Control Nicholas Francis Pietra

By incorporating the chemistry of frustrated Lewis pairs into polymeric materials, we plan to create new methods for gas storage and catalytic applications. The poster discusses the synthesis and characterization of the polymeric Lewis base to be used in the frustrated Lewis pair, Poly(4-diphenylphosphino styrene). The polymer was synthesized by anionic polymerization and characterized using SEC, proton NMR, and 31P NMR, which will be essential in determining adduct formation between the acid and base.

Dr. Robert Hickey 143
3D Full-Field Mechanical Measurement of Shoulder Bones before and after Implant Placement Chujie Gong

This work presents the results of a noninvasive three-dimensional (3D) full-field mechanical measurement of shoulder bones under different loading conditions. Micro X-ray Computed Tomography (µ-XCT) of the specimen is taken under no-load and loaded conditions. Then a glenoid implant is cemented into the specimen by a shoulder surgeon. µ-XCT of implant-bone structure is taken under no-load and loaded conditions.The deformation and strain in bone with or without implant under various loading conditions are compared.

Jing Du 144 Alumni & Heritage Halls, HUB-Robeson Center, University Park Campus
The Dependence on lon in E.coli Suppressor Pathways Alexa Hughes and Paige Laughlin

The prlF1 mutation and overexpression of the genes ptsN and yhbW have been found to allow E.coli to survive when σE is absent. These genes also have the ability to alleviate the effects of lamB-lacZ hybrid jamming in secretion machinery and OmpC overexpression, both of which are toxic. We hypothesize that all of the suppressor pathways that allow for survival when σE is absent, require the lon gene.

Dr. Sarah Ades 145
Determination of the genetic background of Rhesus Macaques Alison Claire Barrett The genetic origin of rhesus macaques, which are important model organisms in medical and biological research, is often not well documented, despite being of high importance in various scientific questions. In this project, we sequenced mitochondrial DNA of rhesus macaques of unknown origin and analyzed a region containing variable sites between Indian and Chinese macaques. This allowed us to determine the genetic background of these monkeys. Kateryna Makova 146
Understanding the Influence of Motivational Readiness on the Relationship between Desired Body Image and Physical Activity in African American Adults Hannah Tripp Jones The purpose of this study was to explore the mediating role of motivational readiness on the relationship between desired body image and moderate-vigorous physical activity (MVPA) in African American adults. We found that as desired body image decreased, motivational readiness for exercise and MVPA increased. Results suggest the importance of increasing awareness of risks associated with obesity in an effort to reduce preferences for a higher body shape and size among African American adults. Scherezade Mama 147
Timing and Impact of Habitat Type on Black Bear Corn Crop Damage Alec Scott Baker Our study compared bear damage to cornfields between forest cover in northern Clarion County (n=5) and agriculture in southern Clarion County (n=5). Damage was surveyed weekly from 13 August 2017 to 22 October 2017. There was strong evidence that surrounding habitat type had a significant impact on percentage of damage (p = 0.016). The difference in mean amount of damage was not significant (p = 0.116) between the two habitat types. Keely Roen 148
Synthesis of a Novel Small-Molecule Inhibitor of D14-type Strigolactone Receptors Sojung Kim Strigolactones are plant hormones that stimulate germination of Striga hermonthica, a major agricultural pest in Africa. A small-molecule called DL1 was discovered to inhibit strigolactone signaling. I studied the structure-activity relationship of DL1, then designed and chemically synthesized over 40 DL1 derivatives to create stronger inhibitors. This ultimately led to the synthesis of a novel inhibitor nearly 4.5x stronger than DL1. This potent inhibitor is now being studied as an agrochemical to control Striga germination. Thomas Mallouk 149
Implementing Urban Green Agriculture in Cuba to Increase Food Production and Agricultural Sustainability Hannah Albright and Alexandra Roque

This research was undertaken as part of a course-based undergraduate research experience (CURE) structured through the 2017 Penn State CHANCE Program in Cuba. Experiential learning in the field allowed for the examination of farming methods as they relate to agricultural sustainability. Green roofs could enhance the quality of living and introduce many benefits to urban settings. It is recommended that Cuba begin to look into constructing green roofs and creating policies that facilitate this practice.

Jacqueline McLaughlin 150
Structural Analysis of the MLL Histone Methyltransferase Complex Rosalie Sowers

The MLL complex is a histone methyltransferase complex that plays a key role in transcriptional activation by covalently modifying the nucleosome. However, our knowledge of its structure and mechanism is limited. We hypothesize that the MLL complex has a precise binding interaction with the nucleosome that can be determined via X-ray crystallography. Solving the structure of this complex bound to its nucleosome substrate will inform us of its mechanism of chromatin regulation.

Dr. Song Tan 151
Distinguishing Between the SigmaE and Hfq Pathway Inhibitors in Escherichia coli Kushagra Aniruddha Kumar, Bryn Kelly and Lindsey Engleman

The goal of this project is to discover new antibiotics that target the SigmaE/Hfq pathway required for virulence and maintaining the integrity of the cellular membrane in Gram negative bacteria such as E. coli. Previously, a library of chemical compounds was screened to discover potential inhibitors of this pathway. Now these potential chemical inhibitors must undergo a series of secondary assays to differentiate whether the compounds inhibit either the SigmaE or Hfq pathway.

Sarah Ades 152
Inhibitors of the SigmaE-Hfq Regulatory Pathway in E. coli Katherine Elizabeth Winner The SigmaE-Hfq pathway found in Escherichia coli. is a good potential target because SigmaE is important for pathogenesis in some bacteria and important for cell survival in others. The SigmaE-Hfq pathway is involved with maintaining the integrity of the cell membrane. This project tests the importance of this pathway in biofilm formation and stress survival of the cell. Sarah Ades 153
Pharmacological Data to Determine the Potential of Helper Compounds for Use as Antibiotics Tianna I Fredericks

Antibiotic resistance is a growing issue that leads to the ineffectiveness of drugs that treat bacterial infections. New investigations are currently underway to determine the effectiveness of helper compounds, which include a range of drug classes, particularly antihistamines. Antihistamines oppose the constitutive activity of histamine receptors, which function to produce biological responses. The antihistamine, Azelastine, possesses antibacterial activity by altering bacterial membrane permeability. 

Dr. Kenneth Keiler 154
TEXT MESSAGING TO ENHANCE A COMMUNITY-BASED HEALTH INTERVENTION IN RURAL SOUTH AFRICA Taylor Beth Haddad South Africa (SA) has dramatically increasing diabetes prevalence due to changes in lifestyle (e.g., diet, exercise), requiring widespread prevention efforts. As part of adapting the Diabetes Prevention Program (DPP) for delivery in rural SA, we developed and implemented text-messaging components to enhance face-to-face DPP programming. We present the development of content (336 health-relevant, culturally appropriate messages) and implementation (delivery frequency, intensity, and scheduling logic). This messaging-enhanced DPP encourages participation and facilitates positive lifestyle change. Joshua Smyth 155
Investigating the Role of the Cytohesin-2/ARNO Coiled-Coil Domain on Epithelial Cell Migration Sanjana S Marikunte Epithelial cells line skin, lung, and digestive tract surfaces.  While normally stationary, epithelial cells migrate during wound healing, cancer metastasis, and early development.  This migration is regulated by small GTPases and stimulated by hepatocyte growth factor.  A multi-protein complex involving two GEFs including cytohesin-2/ARNO and two scaffold proteins must form to activate two GTPases.  It was hypothesized that mutations in the coiled-coil domain of cytohesin-2/ARNO would decrease some protein-protein interactions and inhibit cell migration. Lorraine Santy 156
Development of a Multi-Emitter LED Light Source John Sloane With the rise in popularity of LED lighting, the market is beginning to realize some downfalls. For most LED’s at low light levels, the color rendering quality decreases. There are many applications where enhanced colors when dimming would be appropriate, such as museums and restaurants. The purpose of this research is to investigate the phenomenon of decreased color quality at low light levels, in turn creating a solution using readily available and new technologies. Kevin Houser 157
Lexical Decision and Semantic Relatedness Judgment Processing Erika Exton Numerous studies on semantic memory observed that neurologically healthy monolinguals are faster and more accurate in processing concrete than abstract words. This study examines concreteness effects in lexical decision and semantic relationship judgment tasks in the second language in bilinguals and in the first language in people with aphasia, and the relationship between concreteness and types of semantic relationships. Implications for bilingual semantic memory and comparisons between L2 and impaired L1 will be discussed. Janet van Hell 158
Determining the Contributions of the spp-1 and umps-1 genes on the Hyperactive Muscle Phenotype Yixuan Wang Many diseases involved in muscle function are gene related. The ok2703 mutant strain contains a deletion in parts of the spp-1 and umps-1 gene sequence and shows a hyperactive muscle phenotype in the model organism Caenorhabditis elegans. I plan on using thrashing assays and qrt-pcr to determine the contributions of these two genes to the phenotype. Hopefully, the findings can contribute to identifying an effective method to increase muscle locomotion in people with muscle weakness. Wendy Hanna-Rose 159
Active-Z Printing: A New Way to Improve 3D Printed Part Strength Jivtesh Bineet Khurana

Part Shape and build parameters in 3D Printing play a vital role in determining mechanical properties of 3D printed parts. Particularly, the use of 2D layers printed parallel to the build surface produces weak parts especially when stresses perpendicular to the layer interface. We investigate a novel approach that uses non-planar 3D layers, Active Z printing, to improve mechanical strength by aligning the principal stresses in the part parallel to the material deposition paths.

Timothy Simpson 16
Role of Librarians in Book Reviews Christian Lucia, Shuvanon Shahid and Cooper Wills

Often overlooked, book reviews can open a doorway for students to work with librarians to help make their work more scholarly and legitimate. What happens as a librarian becomes a key player in writing book review? In our work, we looked at aid of librarians impacts the quality of a book review co-authored by a student. In addition to background research, the research team conducted a first-cut analysis wherein book reviews were individually rank ordered.

Dr. Nicholas Rowland 160
Characterization of Neuron Injury Response Daniel Edward Ferriss, Sophia Llanos Landaeta and Rachel Swope

Neurons must be particularly adept at responding to injury in order to continue to perform their regular functions. In our research we use Drosophila larvae as model organisms for studying response to neuron injury. Specifically, we are studying the pathways involved in the response to dendritic injury and in the axonal neuroprotective pathway, as well as establishing a new method for measuring response.

Melissa Rolls 161
Companions in Color: High-Resolution Imaging of Kepler’s Sub-Neptune Host Stars Austin T Ware Sub-Neptune exoplanets, which fall between 1 and 4 times the size of Earth, were found in abundance by the Kepler Mission. The combination of their size and relatively short orbital period make them unusual in relation to the Solar System, leading to the question of how these exoplanets form and evolve. One possibility is that they were influenced by distant stellar companions. We conduct a search for visual stellar companions to asses their influence. Eric Ford 162
A Delayed Hatching Phenotype in C. elegans is Linked to Disruption in NAD+ Biosynthesis from Nicotinamide Riboside Josef Michael Blaszkiewicz, Julia Catena and Nicholas Cann

We use C. elegans as a model organism to study the impact of NAD+ metabolism on development. We have identified a delayed-hatching phenotype associated with deficiency in the nmrk-1 gene, which is involved in NAD+ biosynthesis. We examined deficiency in the genes nmat-1 and nmat-2, which operate directly downstream of nmrk-1. Through mutant analysis and RNAi knockdowns, we have found that a similar, possibly more severe phenotype is associated with deficiency in the nmat genes.

Wendy Hanna-Rose 163
Developing Effective Policies for Conserving Mangrove Forests in Cuba Erifili Draklelis and Tianna Fredericks

Mangroves are tropical trees that provide numerous economical and terrestrial advantages, such as protection from coastal erosion and pollution removal. Cuba is home to the third highest concentration of mangroves, but anthropogenic sources serve as the largest threat to their development. Based on reviews of primary literature and policy, and key informant interviews with Cuban marine experts, we recommend developing new policies concerning mangrove reforestation while improving upon current policies with efficient changes.

Jacqueline McLaughlin 164
Locational Date Preferences of Couples in the State College Area Michael Cove Citrenbaum Couples in the State College area filled out surveys on their top 10 favorite date spots, resulting in mostly local locations. Most of the respondents were the females in the relationship. The study observes differences in how many couples prefer campus locations as opposed to the downtown and vice-versa. Maps and exploratory statistics help illustrate these findings. There is also supporting demographic data. Clio Andris 165
Characterization of a Novel Neuronal Injury Model in Drosophila Aabha Premal Vora and Mila Tamminga

Neurons must last an entire lifetime, despite facing frequent risk of injury from sources such as traumatic brain injury. Injury response is often modeled in Drosophila by using a laser to sever neurites and is well-studied. However, we recently observed an alternate type of injury, termed explosion injury, which produces an immediate, massive increase in cytosolic calcium and microtubules. Our goal is to characterize this cellular response, including its relationship to calcium and microtubule upregulation.

Melissa Rolls 166
Oxidative Stress Contributes to the Delayed Hatching of nmrk-1 Deficient C. elegans Nicole Grace Laganke, Elizabeth Eberly and Michael DeGennaro

NAD+ is an essential molecule in biological processes such as metabolism and lifespan. Deficiencies in NAD+ lead to complications in the model organism C. elegans. We research a phenotype caused by a gene-environment interaction involving the riboside pathway of NAD+ synthesis. This delayed hatching phenotype occurs when nmrk-1 is knocked down and the animal is grown in oxidative stress environments. We seek to understand the contribution of environmental factors to the expression of delayed hatching.

Wendy Hanna-Rose 167
Examining Mechanisms Regulating Microtubule Polarity in Class I Drosophila Neurons Emily Claire Yanoshak and Mikala Shaffer

Microtubules are important to cell structure and for helping differentiate between dendrites and axons in neurons. The polarity of microtubules is oriented with the axon being plus end out and the dendrite being minus end out. We know several mechanisms that contribute to microtubule polarity in Class 1 drosophila neurons, however we are concerned with examining checkpoint systems at the cell body exit points and mechanisms controlling microtubule polarity after they are nucleated.

Melissa Rolls 168
Investigating Axonal Neuroprotection and Dendritic Response to Injury in Drosophila Neurons Rachel Swope, Daniel Ferriss and Sophia Landaeta

It is crucial that we understand how neurons respond to injury, especially as these cells may be subject to some form of nerve damage. In our work, we used Drosophila larvae as a model organism to investigate the axonal neuroprotective pathway and attempt to identify key proteins in the largely uncharacterized pathway for response to dendritic injury. We have also been working towards establishing a new method for measuring neuronal response to injury.

Dr. Melissa Rolls 169
Stormwater Modeling of Low Impact Development Practices on Campus Matthew Coleman

This research focused on modeling some of the stormwater best management practices on campus in the Stormwater Management Model. This model was used to calculate the efficiencies of some of the practices. It was also used to determine which practices can and should be used for future developments to the campus.

Cibin Raj 17
Psychodynamic Therapy vs CBT for Adults with Major Depressive Disorder Edie Ye This research proposal is designed to determine the efficacy of CBT and psychodynamic therapy for adults with MDD. The number of depressive symptoms will be measured using the BDI. After a screening period, 240 participants will be randomly assigned to four treatment groups. The treatment groups are CBT and medication, CBT only, psychodynamic therapy and medication, and psychodynamic therapy only. All participants will attend psychotherapy once a week for 20 weeks. Nicholas Pearson 170
Protein Localization and Function in Drosophila Neurons Philip P Ratnasamy and Katelyn Rudisill

In the Roll's lab, our group studies protein localization across the different classes of neurons found in the model organism Drosophila. These proteins are heavily implicated in cell function and structure, and lead to specific abnormalities in neuronal cells when they are absent or otherwise manipulated, therefore, studying these proteins will allow for deeper insight into the causes of various neurodegenerative diseases found in humans. 

Dr. Melissa Rolls 171
Exploring the Economic Utilization of Invasive Species to Improve the Select Health of Ecosystems in Cuba Ethan J Liu and Logan Staley

In our recent embedded study abroad program in Western Cuba, we were given the opportunity to learn about sustainability and environmental conservation policy. Our immersion with the Cuban people, flora, and fauna facilitated a deeper intrinsic understanding of fragile ecosystems and their unnatural invasive disruptions. In this case study analysis, we evaluate the economic utilization of two invasive species, the lion fish and marabou shrub, in order to reduce their detrimental impact on endemic species.

Dr. Jacqueline McLaughlin 172
Protein Localization in class I & III neurons in Drosophila organisms Katelyn Emily Rudisill et al

In the Rolls lab we look at protein localization in the model organism drosophila. Proteins have key roles within organisms and are vital for the survival of organisms. Without the proper proteins in the cell neurons lose their ability to properly function in the cell. By using different RNAi fly lines various proteins can be knocked down and the effects it has on the organism can be observed using different microscopy techniques. 

Dr. Melissa Rolls 173
Thermal Transport in 2D Semiconductors Yiwen Song Accurate assessment of thermal properties of MoS2, a two-dimensional layered semiconductor material, is essential for the development of ultra-efficient low power electronic devices based on this material. However, thermal properties of MoS2 are not well understood due to limitations associated with existing experimental techniques. In this study, thermoreflectance thermal imaging and Raman thermometry will be used to obtain accurate data for thermal conductivity calculation. Sukwon Choi 174
Comparison of Science Faculty Committee Workloads Centered on Gender Sarah Fieger This research seeks to identify if gender or other factors play a large role in predicting the committee workload of a faculty member. Specifically, in this study the population was the Penn State University Mathematics and Biology department’s academic service assignments. This research has been conducted with the support and collaboration of both department heads and may inform new departmental policies in the future. Nate Brown 175
Coupling OpenFOAM with external solver Sen Lu OpenFOAM is a powerful open source toolbox software for solving computational fluid dynamics (CFD) problems. While it is powerful enough to excel in the field, running OpenFOAM solver with other physical engines can drastically increase its audience who is interested in game engine, simulation models, etc. We explored two ways of coupling a general program with OpenFOAM using Message Passing Interface (MPI) and evaluated their effectiveness and usability. Xiaofeng Liu 176
Examine the Factors that Lead to Graduate School Jiajin Xiao

Graduate school is crucial for students. In this study, we use Crisp-Set Qualitative Comparative Analysis (csQCA) to examine 50 personal statements from the winners of National Science Foundation to find factors that lead them to graduate school. Student’s family background, undergraduate experience and etc. are some of the factors. This study gives us a better perspective and understanding of the reasons for students to go to graduate school.

Dr. Cathrine Berdanier 177
Time spent performing household tasks and African American adolescents' academic functioning. Nicole A Telfer I examined the association between academic functioning and time spent performing household tasks among 344 African American adolescents. I also tested if the association between academic functioning and time spent performing household tasks differs for boys and girls. Adolescents completed questionnaires about their school experiences, and mothers reported report card grades. Adolescents who spent more time performing household tasks reported more school trouble and lower GPA. These associations did not differ for boys versus girls. Susan Marie McHale 178
Characterization of Novel Delayed Hatching Phenotype observed in Caenorhabditis elegans Vienna Louise Huso and Teniola Idowu

C. elegans containing a knock-out mutation in the nmrk-1 gene do not synthesize an enzyme known as nicotinamide riboside kinase which is involved in the synthesis of NAD+. Preliminary results suggest that a combination of this genetic mutation and an environmental stressor result in a novel delayed hatching phenotype exhibited in eggs laid by nmrk-1 mutants. Our results provide more details about the lifespan of unhatched embryos and the strength observed in this phenotype.

Wendy Hanna-Rose 179
Determining how neural connectivity affects activity through modeling of the Combinatorial Threshold Linear Network. Elena Christine Cadenas

The Combinatorial Threshold Linear Network (CTLN) models neural activity as a network of nodes where activity is determined by binary synapses. The research presented here used a software implementation of the CTLN to analyze long run neural activity resulting from a variety of initial activation states.  The results demonstrate the variability of attractive states that can be triggered in network structures having the same degree sequences.     

Dr. Carina Curto 18
Towards a decision support framework for mitigating the risk of VBDs such as malaria through engaging with built environment professionals Sumit Pareek According to the World Health Organization, there were 216,000 estimated cases of malaria in 2016. The built environment can propagate the spread of malaria. This research uses a web of causation approach and a survey to identify and validate knowledge gaps between the built environment and public health industries. Data is used for providing recommendations on how to leverage decision making during the construction process as a tool to mitigate the spread of malaria. Esther Obonyo 180
Structural improvement of soft network composite for flexible electronic Junghsien Wei   Flexible electronics is a technology of making electronics devices on flexible plastic materials, which offers traditional electronic devices the ability to be extensible and wearable. Our research provides a simple solution to tailor the mechanical stiffness of composite substrates to improving and strengthening the structure. We design our own hex pattern structure, which includes several holes inside in order to make the strain and stress response much higher. Huanyu Chen 181
Creating a software bridge between a car simulator and traffic simulator. Karan Durgesh Shah The primary way to currently test safety-critical driving systems is via driving simulators specially for multi-vehicle Connected and Autonomous Vehicle systems. Currently, it is difficult to scale the virtual reality environment to incorporate virtual traffic that can accurately identify and respond to a human controlled virtual car. The project is aimed to enable two simulators (a virtual car simulator and a traffic simulator) in different software to interact with each other.  Sean Brennan 182

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