Measuring Corticosterone (CORT) can give an overview of animal activity. The study specimen, red-backed salamander, is widely distributed however, little is known about their diurnal corticosterone cycle. We predicted CORT would be highest at dusk for this nocturnal species. Using the water bath method, we noninvasively sampled CORT. In our study, the red-backed salamander’s CORT levels increased around 12AM and peaked at 6AM. Results contradicted our hypothesis and may suggest that the salamanders upregulate digestion.