The interaction between the human body and its gut microbiome is an area of interest in regard to human health. The detection of bacterial lipoproteins by host Toll-Like Receptors is a key part of this interaction. Ruminococcus ganvus is an anaerobic, gram-positive firmicute residing in approximately 90% of human gut microbiomes. This research characterizes the lipoprotein structure in R. gnavus and measures its ability to induce a TLR response in HEK293T cells.