Heart rate variability (HRV) reflects autonomic nervous system activity and has been linked to human performance under stress. Few studies have examined HRV and performance in virtual reality (VR), which simulates naturalistic stress conditions. In the current study, HRV and performance were measured in a VR shooting task under low and high stress. Results indicate correlations between HRV and shooting false alarm rates that are consistent with prior studies and neurocognitive theories of HRV.