The farnesoid X-receptor (FXR), a nuclear receptor, controls bile acid, and glucose metabolism. When ligands bind to the ligand-binding domain, structural changes occur in the DNA-binding domain and alter the DNA binding preferences of the DNA-binding domain. Studies have crystallized the structure of FXR; however, there is debate as to which specific amino acids are responsible for differential binding, which this project aims to investigate through MD simulations, site-directed mutagenesis, and dual-luciferase assays.