Antibiotic resistance is due to the misuse and overuse of antibiotics. New methods of creating antibiotics are needed. Trans-translation is a bacteria-specific process allowing the release of stalled ribosomes and is a potential antibiotic target. New trans-translation inhibitors have been found to alter the conformation of the ribosomal protein L27. Deletions made at the N-terminus of L27 determine the protein and inhibitor interaction. Findings suggest changes in inhibitory concentrations among inhibitors, indicating different L27 interactions.