While dam removal has accelerated across the US in the interest of reducing human safety hazards and restoring stream integrity, few studies have evaluated long-term effects. We selected the former McCoy-Linn Dam site (Spring Creek, Centre County, Pennsylvania) to analyze long-term response of habitat and benthic macroinvertebrate assemblages. Outcomes of this study will help inform monitoring programs that seek to evaluate the effects of dam removal on stream ecosystems.