Probiotic bacteria are anti-inflammatory and are typically absent in chronic illnesses. One probiotic, Bifidobacterium, is prevalent in the colon microbiota and abundant in infants. Colon lining epithelial cells have immune system toll-like-receptors (TLR) 2s that can detect bacterial lipoproteins, causing inflammation. It is unknown if Bifidobacterium stimulates TLR 2’s in the colon due to net pro-inflammatory properties. Investigating the structure and TLR response to Bifidobacterium’s lipoproteins will provide insight into understanding lipoprotein-immune system interactions.