H-NS and CRP are key regulators of gene expression in bacteria, influencing responses to environmental changes. This study investigates their potential interactions with 2’,3’-cNMPS, small molecules implicated in bacterial stress responses. By analyzing the expression and binding of H-NS and CRP in the presence of 2’,3’-cNMPS, we aim to uncover new regulatory mechanisms. Understanding these interactions could provide insights into bacterial physiology and open new ideas for controlling gene expression in prokaryotic systems.