Trans-translation is the main ribosome rescue pathway for bacteria that removes ribosomes stalled on mRNAs during translation. Since trans-translation is ubiquitous in bacteria but not conserved in humans, trans-translation inhibitors have great potential to address the urgent need for new antibiotics able to treat MDR bacterial species. The goal of the proposed research is to determine whether trans-translation inhibitors could be used as an alternative treatment for infections caused by gram-positive pathogens