The Kinetochore connects centromeric chromatin to microtubules during mitosis. It was recently discovered that kinetochore proteins also function in post mitotic neurons, where they regulate microtubule nucleation. To test the hypothesis that centromeric chromatin is also important in post mitotic neurons, genes in centromeric DNA will be knocked down by RNAi and microtubule behavior will be analyzed. So far, at least one gene in centromeric DNA is required to regulate microtubule dynamics.