Batrachochytrium dendrobatidis (Bd) is the species primarily responsible for chytridiomycosis, which affects amphibians on multiple continents. Amphibians have a mucus layer that contributes to the animal's immunity. The standard methods for measuring mucosome function involves placing individuals in a water bath for 1 hour. Reducing collection time has a clear advantage. By examining the collection methods to determine if shorter collection times are effective we can increase field work efficiency and minimize animal stress.