Monotropa uniflora (ghost pipe) is a parasitic plant traditionally used for pain relief, though its bioactive compounds remain largely unidentified. Chronic pain, affecting approximately 20% of the U.S. population, often leads individuals to seek complementary therapies due to the adverse effects of pharmacological treatments. This study aims to evaluate the cytotoxicity of ghost pipe extracts using in vitro bioassays. Statistical analysis (one-way ANOVA, Tukey’s test) will identify bioactive fractions for subsequent metabolomic analysis, potentially revealing compounds with therapeutic potential.