Antibiotic resistance is an ever-growing problem in society. In order to combat this, the development of new drugs with novel targets is essential. The aim of this research is to identify inhibitors of the Sigma E/Hfq pathway in E. coli through primary screening and confirmation via secondary assays. This pathway is involved in maintaining cell envelope integrity and is necessary for E. coli viability, which validates this pathway as a good target for drug development.