Crystalline silica (cSiO2) has been found to be a toxic trigger for autoimmune diseases, such as lupus, when inhaled for an extended period of time. The cSiO2 is phagocytosed, causing the release of cytokines and eventually cell death. In order to examine this pathway in-vitro, RAW264.7 macrophages were stimulated by lipopolysaccharides before the cytotoxicity was measured through a lactate dehydrogenase (LDH) assay. Through the LDH assay, a dose-dependent cSiO2 toxicity was observed.