Since the initial discovery of catalytic RNAs, or ribozymes, various examples such as the twister ribozyme have been discovered. We hypothesized that part of the twister catalytic mechanism could be studied in isolation via RNA hexamers that were shown to cleave at UA or CA motifs, similar to twister. I found that these UA and CA motifs in small hexamers do not possess intrinsic reactivity as originally observed, illustrating the importance of twister’s 3D structure.