Plasmodium parasites are the causative agent of malaria.These parasites contain a conserved tudor staphylococcal nuclease (TSN) whose function is not well understood; our lab has previously demonstrated that PyTSN1 interacts with translational repressors involved in the preparation of parasites for transmission to mosquitoes. To better characterize this protein, I have generated three transgenic parasite lines to identify function, localization, and protein-protein interactions of PyTSN1 through gene deletion, tagging, and proteomic approaches.