A previous microbiome study found significant associations between Listeria monocytogenes (LM), a foodborne pathogen, and the fungal family Dipodascaceae, in apple packing facilities. The goal of this study is to isolate and identify Galactomyces spp. in the family Dipodascaceae from apple packing facility environments and investigate the effect of metabolites produced by Galactomyces spp. on the growth of LM. Our results suggest that time-dependent production of Galactomyces-associated metabolites influence the ability to suppress LM growth.