Fatty acid kinases A and B are involved in lipid metabolism and synthesizing membrane phospholipids in gram-positive pathogens like Staphylococcus aureus. This research aims to determine the effects of FakA and FakB deletions on their potential involvement in adding an acyl chain to lipoproteins in S. aureus as well as variations in toll-like receptor activation. This may also contribute to revealing the dynamics of the bacterial cell envelope and developing techniques to counter antibiotic resistance.