Sepsis occurs when the immune system responds to an infection in the bloodstream; this often leads to organ damage, organ failure, and death. Successful treatment of sepsis relies on precise diagnosis of  bacteria. Emerging technologies, such as microfluidic devices, offer huge potential for transforming diagnosis of sepsis. This project, in specific, seeks to design a microfluidic device that can isolate and characterize bacteria at sepsis-level concentrations with species, such as Escherichia coli and Staphylococcus aureus.