Characterization of HelD, a Novel RNA Polymerase Binding Protein in Bacillus subtilis

by Uyen Nguyen

The goal of this project is to obtain a high-resolution three-dimensional structure of the protein HelD in complex with B. subtilis RNAP to elucidate the structural basis of RNAP recycling by the DNA helicase HelD. Two techniques have been used toward the structure determinations: X-ray crystallography to determine the structure of HelD, and single-particle cryo-electron microscopy (cryo-EM) to determine the structure of HelD-RNAP complex. Overall, this project provides a framework for purification protocol of HelD.

Major: 
Biochemistry and Molecular Biology
Exhibition Category: 
Health and Life Sciences
Exhibition Format: 
Poster Presentation
Campus: 
University Park
Faculty Sponsor: 
Dr. Katsuhiko Murakami
Poster Number: 
277