Petunia inflata uses a mechanism named self-incompatibility to prevent inbreeding. Self-incompatibility allows the plant to reject genetically identical (self) pollen yet accept genetically dissimilar (non-self) pollen. Self/non-self recognition is determined by the polymorphic S-locus. To determine amino acids involved in interactions between SLFs and S-RNases, chimeric genes of S2-SLF1 and S3-SLF1 were introduced into P. inflata. I have also tested whether S2-SLF2, S