In order to understand the self-interaction of spectrin, a construct was designed by Dr. Seung-kyu Lee that was recombined into Drosophila melanogaster to quickly and efficiently mutate the C-Terminus of spectrin to see how each mutation affects spectrin’s function. The purpose of this experiment was to see if this construct was successfully inserted into the genome and, if not how much of the construct was preserved, or where it may have ended up.