by Phillip Ryan Clauer
Riboswitches can detect small molecules and serve as sensitive biological diagnostics. However, engineering riboswitches for use in diagnostic applications requires the design of genetic terminators compatible with viral polymerases. We report on an in vitro assay using cell-free protein synthesis lysates capable of quantifying termination efficiency to screen terminator candidates. We also demonstrate our assay measurements were consistent with in vivo results and that in vitro conditions can be optimized to dramatically increase terminator efficiency.
Major:
Biochemistry and Molecular Biology
Exhibition Category:
Engineering
Exhibition Format:
Poster Presentation
Campus:
University Park
Faculty Sponsor:
Julius Lucks, Associate Professor
Poster Number:
215