Micro-particle image velocimetry (μPIV) provides the ability to quantitatively measure microflow alongside thrombus development. Using a μPIV platform, we aim to capture the real time in vitro formation of a thrombus and better understand the micro-scale factors related to thrombus growth. Preliminary data support the μPIV system's ability to quantitatively measure flow. Results were compared to a computer COMSOL model under the same flow conditions. The experimental data validated the computational model.