White button (WB) mushrooms have been reported to have health benefits, through poorly described mechanisms. Our data showed that WB feeding (1%) resulted in higher levels of cecal succinate and the succinate-producing bacteria Prevotella. Microbial succinate production induced expression of genes (G6Pase, Glut2, FFAR3, and SGLT3) important for intestinal gluconeogenesis (IGN) via the gut-brain neural circuit. Germ-free mice had no such WB mediated effect.