The objective of this study is to create engineered protein variants, in order to determine how the reaction outcomes in Fe-2OG-dependent enzymes are decided. One such enzyme, hyoscyamine-6β-hydroxylase, possess both hydroxylase and epoxidase functions. Depending on the variant’s properties, the outcome is either an increased hydroxy product or a secondary dihydroxy product. Screening the products is done two ways: whole-cell reactions to determine initial hits and purified enzyme reactions to verify their activity.