Epithelial cells line skin, lung, and digestive tract surfaces.  While normally stationary, epithelial cells migrate during wound healing, cancer metastasis, and early development.  This migration is regulated by small GTPases and stimulated by hepatocyte growth factor.  A multi-protein complex involving two GEFs including cytohesin-2/ARNO and two scaffold proteins must form to activate two GTPases.  It was hypothesized that mutations in the coiled-coil domain of cytohesin-2/ARNO would decrease some protein-protein interactions and inhibit cell migration.