We studied the drosophila melanogaster homolog to TMEM132a, named detonator (dtn). Using RNAi and TMEM132a-deficient fly lines, we bred offspring with appropriate driver lines to produce offspring with reduced dtn expression, indicated by lethality or a balancer chromosome-induced phenotype. We found that dtn knockdown resulted a reduction in whole wing size in flies bred with MS-1096 driver lines. Dtn knockdown resulted in lethality, as shown by reduced numbers of male offspring in tub-Gal4 driver crosses.