Antibiotic resistant bacteria are a multifaceted problem threatening the world’s population and require constant research and development. The Rcs Phosphorelay is a conserved pathway among enteric pathogens and shows promise as a potential antibiotic target. This research aims to determine the role of the Rcs Phosphorelay in intrinsic antibiotic resistance. This was achieved through resistance profile comparisons of Cefsulodin resistant isolates to various beta-lactam antibiotics and kill curve analysis with Cefsulodin.