Protein overexpression can lead to dramatic imbalances in the fragile physiological environment. In order to provide effective treatment, it is crucial to identify protein blockers and develop novel delivery methods. DNA aptamers are an emerging class of protein blockers capable of rivaling traditional antibodies. Aptamers can inhibit the bioactivity of the proteins by blocking binding sites or sequestering free protein. This research aims to demonstrate that aptamer-functionalized microneedles possess inhibitory effects on a model protein.