Using Fusion Constructs to Map the Membrane Topology of Lit Protein

In 2017 the Meredith Lab discovered Lit, a putative membrane protein, that is responsible for creating lyso-form lipoproteins. Lit functions as a novel mechanism that has yet to be observed and is critical for understanding how these lipoproteins are formed. This proposed research measures activity of fusion proteins in order to determine the membrane topology of E. faecalis Lit. Results will provide a more in depth characterization of Lit and how lyso-form lipoproteins are made.