With the rapid development of antibiotics came an era of pathogenic bacteria that evolved resistance. This is motive for exploration of an alternative protein control pathway that can be targeted for antibiotic development and surmount these multi-drug resistant pathogens. The pathway exploited in this investigation is trans-translation. My research initiates the investigation of the Tetrazole class, which inhibit this pathway. Major methodology includes MIC, CFU/mL, endospore germination, cytotoxicity assay, and growth curve.