Shroom (Shrm) is a highly conserved family of proteins that are defined by the conservation of actin and Rho kinase binding domains. Shroom family proteins have been implicated in the development of the vertebrate ear, kidney, and in brain connectivity. Little is known about what activates Shrm3 function or localization. To better understand these processes we aimed to find novel protein interactors of Shrm3. The screens returned a number of novel interactors.