Antibiotic resistance is a growing problem in global health, as opportunistic pathogens are causing fatal infections that are unable to be treated. Fatty acid kinase A and B are involved in lipid metabolism and the synthesis of membrane phospholipids in gram positive bacterial pathogens like Staphylococcus aureus. Determining the effects of FakA and/or FakB deletions can prove critical to understanding the dynamics of the bacterial cell envelope and developing new techniques to counter antibiotic resistance.