White nose syndrome is responsible for the death of over 5 million bats and is caused by the fungus Pseudogymnoascus destructans (Pd). Pd is infected with a novel partitivirus, Pseudogymnoascus destructans partitivirus (PdPV). Pd isolates from Pennsylvania often have an additional virus, Pseudogymnoascus destructans quintipartite virus (PdQV). To test the effect of PdQV on PdPV, Pd strains with and without PdQV were grown in culture and the levels of PdPV RNA were compared using q-RTPCR.