Understanding nutrient behavior in forest ecosystems is of paramount importance in proper forest management. This study explores possible mechanisms creating long-term change of forest soil nutrients, namely mycorrhizae types and root thickness, using 6 tree species from a common garden in central Pennsylvania. Analysis showed significant variation between species in soil nutrient contents, and soil carbon, nitrate, ammonium, and pH of arbuscular mycorrhizal species were significant higher than ectomycorrhizal species.