The SigmaE-Hfq pathway is responsible for maintaining the integrity of the cell membrane under cell stress which is important for virulence or viability in gram-negative bacteria. Hfq is a chaperone that helps regulate outer membrane protein mRNAs and is required for biofilm formation. Small molecule inhibitors of the SigmaE-Hfq pathway identified from a high-throughput screen were tested for inhibition of biofilm formation and growth. Future experiments will identify Hfq inhibitors using cell-based fluorescence screens.