NeuroD1 is a neural transcription factor which can be used for conversion of reactive astrocytes into neurons for stroke and Alzheimers disease treatment. NeuroD1 binds to chromatin and facilitates binding of other transcription factors to promote neuronal development. We would like to investigate the mechanism of reprogramming of astrocytes to neurons by determining where NeuroD1 binds. We determined that NeuroD1 binds to Hes6 promoter along with NeuroD1's own promoter through Immuno-precipitation assay.