Nicotinamide adenine dinucleotide (NAD+) is a molecule used as a coenzyme for a variety of metabolic pathways. Our lab has made the discovery that a mutation in an NAD+ biosynthetic pathway that leads to lower levels of the molecule, when coupled with increased oxidative stress leads to a delay in the hatching of C. elegans eggs. This study examines differences in the physical appearance of the eggshells of worms that display delayed hatching.