The axon initial segment (AIS) is a crucial region in neurons required for the generation of action potentials. The scope of this research aims to elucidate the localization of certain potassium and calcium channels in the AIS and determine what anchoring proteins may play a role in their localization. The gating properties of the voltage-gated Elk1 potassium channel are also looked at to determine the functionality of the channels in the AIS.