Sirtuins are a class of NAD+ dependent enzymes that regulate biochemical pathways through mono-ADP ribosylation and various deacetylation reactions. Recently in our lab, knockout of either mitochondrial sirtuins, sir-2.2 or sir-2.3, had an observed lifespan extension of more than 25% on OP50 E. coli. However, lifespan extension may be diet-dependent and needs to be further analyzed on varying food sources to better characterize the phenotype.