Triple-negative breast cancer (TNBC) is an aggressive form of breast cancer with few treatment options. Therefore, we tested if cryoablation of TNBC can induce immune responses using a mouse model. Cryoablation triggered robust increases of Th1 and Th2 cytokines in both spleen and lymph nodes. These immune responses might explain why animals treated with cryoablation, though having circulating tumor cells at the time of treatment, had fewer metastatic foci compared to other forms of treatment.