Promega PowerSeq Whole Mitochondrial Genome System

by Dana Dirnberger
We tested the Promega PowerSeq Whole Mitogenome System, sequencing the entire mitochondrial genome. We sequenced five samples from each population, European, African, Asian, and Latino, totaling twenty. Sequencing yielded concordant or more specific haplogroups for all samples. Coverage assessment showed no drop out regions for any samples, indicating the kit performed well with all populations. The 16114-16277 base pair region showed significant coverage differences between populations, indicating different primer success within populations in that region.
Major: 
Forensic Science, Biology
Exhibition Category: 
Physical Sciences
Exhibition Format: 
Poster Presentation
Campus: 
University Park
Faculty Sponsor: 
Mitchell Holland
Location: 
Heritage Hall, HUB-Robeson Center
Poster Number: 
307