A review for high-pressure freezing preparation of C. elegans tissue for electron microscopy

by Kendel Reed

I performed high pressure freezing for TEM of C. elegans eggshells. HPF is the only fixation available to study EM of C. elegans and results are often more variable. My poster will describe the procedure using the Leica EM HPM100 and the tissue embedding process. The main issue faced during the preparation was extracting fully intact worms from the HPF sample carriers. I conclude with a solution to a more effective approach when embedding samples.

Major: 
Biological sciences and health professions
Exhibition Category: 
Engagement Experience
Exhibition Format: 
Engagement Experience
Campus: 
University Park
Faculty Sponsor: 
Gang Ning
Location: 
Alumni Hall
Poster Number: 
14
Type of Engagement Experience: