Bacteria continuously become resistant to current antibiotics; thus, scientists must continuously synthesize new antibiotics. KKL-896 inhibits a bacterial pathway called trans-translation; however, its molecular target is unknown. To discover the target, resistant mutants were created and whole-genome sequencing of these mutants led to five genes of interest. Each gene has been studied in hopes of defining KKL-896's target. By knowing KKL-896’s target, drug development may advance, ultimately unveiling a new way to combat tuberculosis.