Although iron regulation and transport are well studied, the mechanisms of iron delivery from the transporter to iron-requiring enzymes remains poorly understood. BOLA2 is hypothesized to be an iron-transporting protein within human cells. To provide insight into potential metal-binding interactions, BOLA2 was purified and spectrophotometric titrations of the protein were performed using cobalt(II) as a surrogate for iron(II). The titrations indicate that BOLA2 binds cobalt(II) and motivate further studies of iron(II) binding to the protein.