Bacterial lipoprotein modification is a process that is vital to both Gram-negative and Gram-positive microbial pathogens. Lipoproteins are pathogen-associated molecular patterns that interact with the Toll-like receptor 2 (TLR2) during the innate immune system response. In certain Firmicutes, external copper triggers N-terminal modifications of lipoproteins that can alter TLR2 detection. Herein, we examine the effect of these modifications on copper resistance in the nosocomial pathogen, Entercoccus faecalis.