Previous work has shown that changes in pH are able to regulate the channels of the Ether-a-go-go (EAG) family. Specifically, the Eag1 K+ channel has been explored by applying pH changes to different mutations that were manufactured in the voltage sensor and pore region. Of these mutated variants of the Eag1 channel, two exhibited a gain-of-function and three exhibited a loss-of-function. This work has increased the knowledge of the gating-mechanism of the channel.