Proposed Mechanism for Conversion of 4-chloro-lysine into 4-chlor-allyl-l-lysine via BesC

by Carolyn Telfer
The terminal alkyne amino acid β-ethynylserine (βes) is produced by Streptomyces cattleya (Sc). In our research, we sought to determine the mechanism for the conversion of 4-chloro-L-lysine into 4-chloro-allyl-L-lysine by the enzyme BesC on the pathway to βes. We coupled serial dilutions, varying iron, enzyme and substrate, with liquid chromatography-mass spectrometry (LC-MS) for analysis. Our experimental results showed products for the complete BesC reaction coupled with the preceding enzyme in the pathway, BesD.  
Major: 
Chemistry
Exhibition Category: 
Physical Sciences
Exhibition Format: 
Poster Presentation
Campus: 
University Park
Faculty Sponsor: 
Joseph Bollinger
Poster Number: 
15966